
Design of the six acceptor-stem mutants. The mutants are abbreviated as mutant 1, M1, etc. E. coli-specific bases are in solid font, and yeast-specific bases are in outline. Note that all yeast tRNAAsp genes were designed so that the tRNA contains the E. coli C32 and A37 nucleotides (see text). Modified bases in the cloverleaf structures are not indicated.










