FIGURE 1.
(a) Phe-tRNA bound to EF-Tu: GDP (red) on the 70S ribosome (50S subunit, blue, 30S subunit, yellow). The complex has been stalled with kirromycin just after GTP hydrolysis but before EF-Tu rearrangements. (b) Ribbons representation of the Phe-tRNA (purple) and EF-Tu (orange) inside the semitransparent cryo-EM density of the ternary complex. The beads on the tRNA label mutations that alter the accuracy of translation (dark and light green) and sites of reporter fluors used in kinetic experiments (blue). (DC) decoding center; (GAC) GTPase-associated center.
