A twisted tRNA intermediate sets the threshold for decoding
- 1Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
- 2Howard Hughes Medical Institute, Health Research, Inc., Albany, New York 12201-0509, USA
- 3Department of Biomedical Sciences, State University of New York, Albany, Albany, New York 12201-0509, USA
Abstract
Putting together consistent cryo-EM structure, transient kinetic and mutant tRNA suppressor data, it appears that a deformed or waggling aminoacyl-tRNA is the critical transitional structure examined by the ribosome during decoding at the A site. The unusual conformation may be required for effective proofreading of the codon–anticodon complex.
Footnotes
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Article and publication are at http://www.rnajournal.org/cgi/doi/10.1261/rna.2184703.
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- Accepted January 9, 2003.
- Received November 21, 2002.
- Copyright 2003 by RNA Society
