Two reactions of Haloferax volcanii RNA splicing enzymes: Joining of exons and circularization of introns

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FIGURE 6.
FIGURE 6.

Analyses of splicing products of truncated elongator pre-tRNAMet by different concentration gels. Reactions similar to those shown in Figure 5C using [α-32P]CTP-labeled transcripts were resolved on 20-cm long, denaturing 15% (A) and 20% (B) gels. Lane 1, endonuclease products; lane 2, ligase products. (P) Precursor; (LE) ligated exons; (LI) linear intron; (CI) circular intron; (E) exons.

This Article

  1. RNA 9: 319-330