(A) ADAR2 edits reporter constructs at the R/G site in vivo. Transfection of 293 cells with editing/splicing reporter constructs with or without ADAR2 expression vector. Total RNA was isolated and RT-PCR products corresponding to either spliced or nonspliced RNA were sequenced. The top left sequence corresponds to a nonedited R/G site, indicated by an arrow. Cotransfection with ADAR2 expression vector results in editing seen as a mixed A and G peak at the R/G editing site in the top right and bottom chromatograms. (B) GRG SI and 988 pre-mRNAs were inefficiently spliced in vivo, whereas GRG SS RNA was only detectable as spliced mRNA. The degree of splicing was not affected by coexpression of ADAR2. RT-PCR products corresponding to pre-mRNA and spliced mRNA were separated on 2% agarose gel. Used for size determination was 1 kb PLUS marker (M) (Life Technologies™).
