Efficient splicing and editing of the GRG SS pre-mRNA. (A) In vitro splicing of GRG SS pre-mRNA and GRG SS pre-mRNA lacking the ECS (Δ20). Splicing products were separated on denaturing polyacrylamide gel. The position of nonspliced pre-mRNA and spliced mRNA is indicated to the right of the gel; exons are represented by boxes and introns by lines. The percentage of splicing is indicated below the gel. (B) Diagram of in vitro splicing efficiency of GRG SS wild type and Δ20 pre-mRNA. Splicing efficiency was plotted against time. Data represents three independent experiments, error bars show standard error of the mean, SEM. (C) GRG SI and GRG SS pre-mRNAs can be edited in vitro by recombinant rADAR2a (A2) as shown by limited primer extension. Position of primer and products corresponding to nonedited, R/G, and -1 edited pre-mRNAs are indicated. Numbers below show the amount of editing in percentage in the different substrates.
