(A) Schematic representation of a part of rat glutamate receptor subunit B pre-mRNA. Exons are represented by boxes and introns by lines, not drawn to scale. The R/G editing site and editing complementary sequence are shown in detail. Exonic sequences are written in capitals and intronic sequences in lowercase. The sequence deleted in the Δ20 mutant is underlined. (B) The editing/splicing reporter constructs used in the study. All constructs contain exon 13 including the R/G editing site and the 5′ part of intron 13. GRG 988 contains full-length intron 13 and exon 14, whereas GRG SI has a shortened intron. GRG SS contains part of an intron, the 3′ splice site and exon from a modified adeno major late transcript.
