Edc proteins stimulate recombinant decapping activity. (A) Decapping reactions were carried out in a 15-μL reaction with Edc1p (8 fmoles), Edc2p (8 fmoles), or BSA present. In these reactions, a lower concentration of Dcp1p/Dcp2ΔCp was used to allow the stimulation of activity by Edc proteins to be visualized. The first panel is a PEI cellulose analysis of the reaction products generated after the indicated incubation time. (−) Reaction with no enzyme present. (B) A graphical representation of the data reactions with Edc2p (solid triangles), Edc1p (open circles), and no Edc protein but BSA (solid circles) with percent product plotted against time.
