Decapping enzyme prefers longer substrates. A graphical representation of the activity observed with recombinant Dcp1p/Dcp2ΔCp decapping activity and different-sized MFA2 substrates. The sizes are indicated on the graph: 343 nucleotides (solid circles); 99 nucleotides (solid squares); 29 nucleotides (solid triangles); 20 nucleotides (open circles); and 1 nucleotide (cap analog, m7GpppG) (open triangles). Reactions were carried out as described in Materials and Methods with 1 fmole of the indicated MFA2 substrate, 10-μL reaction and 20 ng total protein. Products were separated by PEI cellulose TLC and quantitated with a Molecular Dynamics PhosphorImager.
