FIGURE 2.
Decapping activity requires a normal Nudix motif in Dcp2ΔCp. Decapping assays analyzed the activity of purified protein fractions where Dcp2ΔCp is wild type or has the E153Q mutation. Dcp1p is present and wild type in all these protein preparations. Decapping reactions were carried out with 1 fmole MFA2 substrate in a 10-μL reaction and ∼225 ng of total protein in the purified fractions. Products were separated by PEI cellulose TLC. (−) Reaction with no enzyme present.
