Analysis of recombinant yeast decapping enzyme

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FIGURE 1.
FIGURE 1.

Purified fractions from cells expressing Dcp1p, Dcp2ΔCp, or Dcp1p and Dcp2ΔCp were assayed for decapping activity. Decapping reactions were carried out as described in Materials and Methods in 10 μL reactions with 1 fmole of MFA2 substrate and ∼20–30 ng of total purified protein. Reaction aliquots were taken and the reaction was stopped at the indicated time points; the reaction products were separated by PEI cellulose TLC. (−) Reaction with no enzyme present.

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  1. RNA 9: 231-238

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