TABLE 1.
Kappa (κ) values for analogs of three adenosines in the U1A stem-loop binding region
| Complex 1 | Complex 2 | Complex 3 | Complex 4 | Complex 5 | |
|---|---|---|---|---|---|
| Experiments were performed in at least triplicate and, for some values, data sets were collected on both 5′- and 3′-labeled U1 snRNA to control for labeling effects. Errors represent the range of values seen in replicate experiments. | |||||
| A65 | 1.2 ± 0.3 | 1.3 ± 0.3 | 1.1 ± 0.2 | 1.3 ± 0.5 | 1.2 ± 0.2 |
| 2AP | 0.4 ± 0.3 | 0.7 ± 0.4 | 2.6 ± 0.9 | 3.3 ± 1.5 | 6.0 ± 2.5 |
| DAP | 0.4 ± 0.2 | 1.2 ± 1.0 | 2.2 ± 0.5 | 3.7 ± 1.9 | 5.7 ± 3.5 |
| m6A | 1.1 ± 0.4 | 1.5 ± 1.0 | 1.3 ± 0.3 | 1.5 ± 0.6 | 2.2 ± 1.3 |
| Pur | 1.2 ± 0.7 | 1.0 ± 0.2 | 1.2 ± 0.4 | 1.0 ± 0.2 | 1.3 ± 0.3 |
| A70 | 0.6 ± 0.3 | 0.7 ± 0.2 | 1.0 ± 0.3 | 1.1 ± 0.2 | 1.1 ± 0.2 |
| 2AP | 0.6 ± 0.3 | 0.7 ± 0.4 | 1.3 ± 0.5 | 1.6 ± 0.6 | 2.9 ± 1.4 |
| DAP | 1.0 ± 0.4 | 1.0 ± 0.3 | 2.3 ± 1.0 | 1.6 ± 1.2 | 2.2 ± 1.3 |
| m6A | 0.4 ± 0.3 | 0.7 ± 0.4 | 2.5 ± 0.7 | 3.8 ± 0.9 | 4.0 ± 2.6 |
| Pur | 0.6 ± 0.3 | 0.6 ± 0.2 | 1.9 ± 0.5 | 2.4 ± 1.0 | 5.5 ± 2.8 |
| A77 | 1.0 ± 0.4 | 1.2 ± 0.4 | 1.0 ± 0.2 | 1.0 ± 0.3 | 1.2 ± 0.3 |
| 2AP | 1.5 ± 1.0 | 2.0 ± 1.0 | 1.6 ± 0.5 | 1.5 ± 0.7 | 1.2 ± 0.3 |
| DAP | 0.8 ± 0.3 | 1.2 ± 0.5 | 1.4 ± 0.5 | 1.0 ± 0.3 | 1.4 ± 0.8 |
| m6A | 1.0 ± 0.4 | 0.9 ± 0.2 | 1.1 ± 0.2 | 1.4 ± 0.7 | 1.0 ± 0.4 |
| Pur | 1.3 ± 0.8 | 1.2 ± 0.7 | 1.0 ± 0.2 | 1.2 ± 0.6 | 1.2 ± 0.3 |
