FIGURE 1.
RT-PCR analysis of Mos mRNA in oocytes injected with Mos dsRNA and treated with cordycepin. The RT-PCR assays were conducted as described previously under conditions that reflect changes in the relative amounts of transcript (Svoboda et al. 2000). Oocytes were microinjected with ∼106 molecules of Mos dsRNA and the relative Mos mRNA levels were assayed 20 h later in either untreated control oocytes or cordycepin-treated (2 mM) oocytes; cordycepin was present from the initial time of oocyte collection. Rabbit globin mRNA was used as an external standard to allow comparison of relative differences in Mos mRNA levels (Svoboda et al. 2000).
