FIGURE 1.
miRNPs from mouse and human neuronal cells. Total extracts from MN-1 (mouse) and Weri (human) cells were incubated with either nonimmune mouse serum (NMS) as a control or the anti-Gemin3 antibody (11G9). Immunoprecipitates were treated with proteinase K, and associated RNAs were purified by phenol-chloroform extraction and precipitated. Isolated RNAs were 3′-end-labeled with [5′-32P]-pCp and resolved by electrophoresis on 15% denaturing polyacrylamide gels. Left: molecular weight marker.
