Synthesis of adenosine derivatives as transcription initiators and preparation of 5′ fluorescein- and biotin-labeled RNA through one-step in vitro transcription

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FIGURE 3.
FIGURE 3.

Synthesis of fluorescein and biotin derivatives of AMP by reaction of amino derivatives of AMP with FAM-SE and biotin-SE. (A) Preparation of adenosine 5′-fluorescein- and 5′-biotin-phosphoramidates, and (B) synthesis of N6-fluorescein and N6-biotin derivatives of AMP.

This Article

  1. RNA 9: 1562-1570

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