Quantification of alternatively spliced FGFR2 RNAs using the RNA invasive cleavage assay

The Invader RNA assay accurately reports levels of FGFR2 alternatively spliced RNAs in crude cell extracts. DT3 cells were transfected with the single-exon minigene constructs described in Figure 6, and either purified total RNA (gray bars) or crude cell lysates (striped bars) were analyzed for the presence of U-D, U-IIIb-D (left panel), or U-IIIc-D RNAs (right panel). The percent inclusion of a product among transcripts was calculated as in Figure 6.