TABLE 1.
Summary of affinity selection yields
| H complex | A complex | |
|---|---|---|
| Quantities were determined by scintillation counting and are correspondingly approximate. 1Losses are due to adhesion of the sample to the walls of the 24-well plate and tubes. 2This includes a correction for quenching by the elution buffer. | ||
| Total input | 125 pmole | 185 pmole |
| No. of gradients/pooled fractions | 5/20 | 8/32 |
| Pooled gradient peaks | 73 pmole | 73 pmole |
| Percent gradient peak/total input | 60% | 40% |
| Input for affinity selection1 | 60 pmole | 57 pmole |
| Beads after washing | 34 pmole | 25 pmole |
| Eluted proteins2 | 27 pmole | 22 pmole |
| % eluted/gradient peak | 37% | 30% |
| Left on beads | 7 pmole | 3 pmole |
| Elution efficiency | 80% | 88% |
