TABLE 1.
Primers and amplicon lengths for RT-PCR
| U7 snRNA | dU7-5′: | 5′-GACAACTCATTGAAAATTTTTATTCTCTTTG |
| dU7-3′: | 5′-TATAATTAGAACGGGAACACTCAATGCCTAG | |
| (sequences corresponding to dU7 snRNA are underlined) | ||
| amplicon length: 88 bp | ||
| Histone H3 | dH3-5′: | 5′-GCGAAGCCTACCTGGTTGGTCTCTTCGAAG |
| pre-mRNA | dH3-3′: | 5′-CTCTCTAGAAACAGAAAATTTTATCTCATTGAATCTGG |
| (sequences corresponding to the histone H3 gene are underlined) | ||
| amplicon length: 230 bp | ||
| β-Actin | dAct-5′: | 5′-CCAGCGTATTAATATAATTAATTTATTCCAC |
| pre-mRNA | dAct-3′ | 5′-TTCTTTATCTTGATGTCCTTGTCATGCC |
| (sequences corresponding to the β-actin gene are underlined) | ||
| amplicon length: 234 bp | ||
