FIGURE 6.
Association of Drosophila Lsm10 and Lsm11 with U7 snRNA in mammalian cells. Human 293-T cells were cotransfected with plasmids encoding HA-dLsm10 and HA-dLsm11. Nuclear extracts were subjected to precipitation with magnetic streptavidin beads coated with biotinylated oligonucleotides complementary to human U7 or U1 snRNAs as indicated. The pellets were subjected to SDS-PAGE, and a Western blot was probed with anti-HA or Y12 antibody (revealing Sm B/B′), as indicated on the left of the figure. Input indicates sample of original nuclear extract; beads, precipitation with beads not coated with oligonucleotide.
