FIGURE 5.
Association of Drosophila Lsm11 with Drosophila U7 snRNA and histone H3 pre-mRNA. Nuclear extracts of either untransfected Drosophila S2 cells (lanes 2–5) or S2 cells stably expressing HA-dLsm11 (lane 6) were subjected to precipitation with protein G–Sepharose beads either lacking antibody (lane 2) or coated with the antibodies indicated above each lane. Lane 1, sample of input nuclear extract from untransfected S2 cells. RNA from each sample was extracted and subjected to RT-PCR for Drosophila U7 snRNA (A), histone H3 pre-mRNA (B), or β-actin pre-mRNA (C). M indicates end-labeled HpaII digest of pBR322 used as size marker.
