Phylogenetic conservation of RNA secondary and tertiary structure in the trpEDCFBA operon leader transcript in Bacillus

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FIGURE 4.
FIGURE 4.

Native gel mobility of the B. stearothermophilus trp leader transcript. 5′ 32P-labeled RNA (1–232) was renatured under various salt conditions and fractionated on a 6% native polyacrylamide gel. Lane 1, RNA renatured in TK0M0; lane 2, RNA renatured in TK100M0; lane 3, RNA renatured in TK100M4. Assignments for each RNA species, based on structure mapping under these conditions (see Fig. 5), are indicated.

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  1. RNA 9: 1502-1515

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