TABLE 1.

Plasmids used in this study

	Transgene			Selectable marker
Plasmid (pHD #)	5′-UTR	ORF	3′ UTR	5′ UTR	ORF	3′ UTR	Promoter	Integration locus	Parent plasmid (pHD #)	References^a
^a(1) Biebinger et al. 1997; (2) L. Storm, D. Horn, and C. Clayton, in prep.; (3) C. Hartmann and C. Clayton, unpubl.; (4) Estévez et al. 2001; (5) this study; (6) Irmer and Clayton 2001; (7) Quijada et al. 2002.
^bThese plasmids are similar to those described in Estévez et al. (2001) except that the 3′ UTRs following the RNAi stem-loop sequences are from a VSG locus.
^cThese plasmids are similar to those described in Irmer and Clayton (2001) or Quijada et al. (2002) except that the selectable marker is PAC and the Tet operator sequence is absent.
1313	EP1	2× TetR	ACT, VSG	ACT	BLE	ACT		TUB	449	1, 2
514	EP1	T7 RNA POL	ACT	ACT	NEO	ACT		TUB	514	3
1153^b	EP1	RRP4 RNAi	VSG	ACT	HYG	ACT	EPI^T1	RRNA	1146	4, 5
1154^b	EP1	RRP45 RNAi	VSG	ACT	HYG	ACT	EPI^Ti	RRNA	1146	4, 5
1199	EP1	CSL4 RNAi	ACT	ACT	HYG	ACT	EPI^Ti	RRNA	1146	4
1413^c	EP1	CAT	EP1	ACT	PAC	ΔALD	T7	RRNA	774	5, 6
1344^c	EP1	CAT	ACT	ACT	PAC	ΔALD	T7	RRNA	789, 1034	5, 6
1414^c	EP1	CAT	ep1ΔURE	ACT	PAC	ΔALD	T7	RRNA	775	5, 6
1415^c	EP1	CAT	PGKB	PGKC	PAC	ΔALD	T7	RRNA	869	5, 7
1417^c	EP1	CAT	pgkbΔURE	PGKC	PAC	ΔALD	T7	RRNA	1044	5, 7
1416^c	EP1	CAT	PPDK	ACT	PAC	ΔALD	T7	RRNA	1317	5, 7
1343^c	EP1	CAT	G₃₀C₃₀EP1	ACT	PAC	ΔALD	T7	RRNA	1413, 921	5, 6

A role for the exosome in the in vivo degradation of unstable mRNAs