FIGURE 2.
Plasmid constructs used to study the effects of exosome subunit depletion on mRNA degradation. The elements indicated are carried in a pGEM backbone. Constructs were linearized within the rRNA spacer region before transfection. The mRNAs produced from the CAT-EP1 plasmid are shown beneath the construct. The CAT mRNAs from the constructs with alternative 3′-UTRs are similar; for ep1ΔURE, only one major polyadenylation site was found whereas for the PGKB mRNAs, several were used (Quijada et al. 2002). Probes used for Figures 3 and 4 are shown as dotted lines with circled numbers: (1) CAT probe; (2) EP1 3′-UTR probe; (3) probe specific for longer EP1 3′-UTR.
