FIGURE 2.
Assay of the 5′ cap status of the MFA2pG mRNA during the lag in the YRP881 (pab1Δ spb2Δ) strain. Total RNA samples corresponding to the time points of the transcriptional pulse-chase experiment indicated on the top of the figure were treated with the recombinant 5′–3′ exonuclease Xrn1p in the presence or absence of the EDTA, as indicated above the lanes. RNA in the lane marked Ao was deadenylated by the oligo(dT)/RNaseH treatment. Sequential Northern blot hybridizations with the MFA2pG (top) and 7S rRNA precursor (bottom) specific probes are shown.
