FIGURE 2.
PNA inhibition experiments. DiGIR2 templates were transcribed in the presence of PNA1488, PNA1283, or PNA1487 complementary to the 3′ SS or to the L2.1 (see Fig. 1A). Different amounts of PNA were included with the transcription reaction (0, 1, 2, 5, 10, 25 pmole/10 μL) and the transcripts analyzed on 8 M urea/5% polyacrylamide gels. The positions in the gels of the relevant RNA species are indicated. Note that the DiGIR2 used in these experiments has a longer 3′ exon compared to that used in other experiments.
