FIGURE 1.
Selection scheme used to evolve the ATP aptamer to MBGT/GTP aptamers. (A) ATP aptamer secondary structure. (B) ATP attached via C8 to agarose. (C) MBGT attached via the morpholine-N to agarose. (D) The initial DNA library contained a 40-nucleotide ATP-aptamer region mutagenized at 15% per position, flanked by constant sequence regions. Transcribed RNA was applied to the MBGT affinity resin for aptamer selection. Bound RNA eluted with GTP was amplified by reverse transcription–PCR, and the resulting DNA was transcribed to yield RNA for the next round of selection.
