FIGURE 2.
(A) Secondary structure of the minimal DCloop hairpin construct used for NMR studies using the numbering convention from HPwt. (B) 500 MHz 2D 1H-1H TOSCY spectra (50-msec mixing time) comparing H5-H6 correlations for HPwt (black), HPdc (blue), and DCloop (red) RNAs at 20°C. Asterisks (*) are used to indicate H5-H6 correlations from the poly-pyrimidine tract nucleotides. (C) First derivative UV melting profile of the DCloop hairpin (200 mM NaCl), with every 10th data point obtained at 260 and 280 nm shown as blue and red circles, respectively. The calculated fit to the data is indicated by solid (260 nm) and dashed (280 nm) lines.
