Inhibition of Escherichia coli RNase P by oligonucleotide directed misfolding of RNA

Inhibition of processing of B. subtilis pre-tRNA by addition of m(CAGCCUAC CCGG) to full-length, renatured RNase P RNA. (A) Autoradiogram of a gel in which m(CAGCCUACCCGG) was added after RNase P RNA was renatured. (Left) m(CAGCCUAC CCGG) and RNase P protein added after renaturation; (right) m(CAGCCUACCCGG) added after renaturation without RNase P protein. (B) Plot of the percentage of pre-tRNA (open circles) and processed tRNA (filled circles) as a function of [m(CAGCCUACCCGG)]. All points have error bars, although in some instances they are smaller than the data points.