Demonstration of the role of the DnaK chaperone system in assembly of 30S ribosomal subunits using a purified in vitro system

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FIGURE 2.
FIGURE 2.

Western blot analysis of Escherichia coli extracts for the DnaK chaperone system. Control, 0.5 μg of purified protein (DnaK, DnaJ, or GrpE) purchased from StressGen Biotech. S100, 20 μg S100 extract; S150, 20 μg S150 extract. (Lane 1) Membrane probed with monoclonal anti-DnaK antibody. (Lane 2) Membrane probed with polyclonal anti-DnaJ antibody. (Lane 3) Membrane probed with polyclonal anti-GrpE antibody. All antibodies were purchased from StressGen Biotech. S100 prepared as reported in (Maki et al. 2002). S150 prepared as reported by Nierhaus (1990).

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