Demonstration of the role of the DnaK chaperone system in assembly of 30S ribosomal subunits using a purified in vitro system

  1. JENNIFER A. MAKI1,
  2. DANIEL R. SOUTHWORTH2, and
  3. GLORIA M. CULVER1
  1. 1Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
  2. 2Johns Hopkins University School of Medicine, Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Baltimore, Maryland 21205, USA

Abstract

Recently, there has been controversy regarding the ability of the DnaK chaperone system to facilitate Escherichia coli 30S subunit assembly at otherwise nonpermissive conditions. Here, we present additional data indicating that purified DnaK chaperone assembled 30S subunits are functional. Additionally, explanations for reported differences are discussed.

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  1. doi: 10.1261/rna.5139703 RNA 9: 1418-1421 Copyright 2003 by RNA Society

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