FIGURE 7.
The association of CF Im subunits with U1 snRNP is not due to RNA tethering. Nuclear extracts were either mock treated (No RNase) or ribonuclease A treated (RNase) and then serially immunoprecipitated, first using anti-U1A mAb 1E1. The supernatant was then precipitated with anti-snRNP Y12. Precipitates were separated by 10% SDS-PAGE and subjected to Western analysis probing with antibody against the 68-kD subunit of CF Im (top panel) mAb 2.73 (middle panel), and 1E1 (bottom panel). The positions of significant proteins are indicated.
