FIGURE 4.
Association of CF Im subunits with snRNPs. Unlabeled nucleoplasmic extract was fractionated on a 5%–30% gradient. Every other fraction was immunoprecipitated with mAb 2.73 as in Figure 2. The precipitates were then separated by 10% SDS-PAGE and subjected to Western analysis sequentially probing with anti-CPSF 160 (top panel), mAb 2.73 (second panel), anti-CF Im 68 (third panel), and Anti-U1A (mAb 12E12; bottom panel). The Westerns were stripped between each probing; however, there was some carryover. The positions of significant proteins are indicated as well as the position of the immunoglobulin heavy chain (hc).
