RNA–protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex

TABLE 2.

Yeast strains used in this study

Strain Genotype Source
aYBZ-1 genetic background.
bK699 genetic background.
cK4452 genetic background.
dRS453 genetic background.
YBZ-1 MATa, ade2, his3-200, leu2-3, 112, trp1-1, ura3-52, LYS2::(LexAop)-lacZ, LexA-MS2-MS2 coat (N55K) Zhang et al. 1997
PJ69-4a MATa his3-200, leu2-3, 112, trp1-90, ura3-52, gal4, gal80, GAL2-ADE2, LYS2::GAL1-HIS3, met2::GAL7-lacZ James et al. 1996
K699 MATa, ade2-1, his3-11, leu2-3,112, ura3, trp1-1, ho, can1-100 Jansen et al. 1996
K4452 MATα, ade2-1, leu2-3, trp1-1, ura3, HO-ADE2, HO-CAN1 Jansen et al. 1996
RS453 MATa, ade2-1, his3-11,15, leu2-3,112, trp1-1, ura3-52, can1-100, GAL+ Segref et al. 1997
YLM585a MATa, ade2, his3-200, leu2-3,112, trp1-1, ura3-52, LYS2::(LexAop)-lacZ, LexA-MS2-MS2 coat (N55K), she2::kanMX Long et al. 2000
YLM777b MATa, ade2-1, his3-11, leu2-3,112, trp1-1, ura3, ho, can1-100, she2Δ silent This study
YLM851c MATα, ade2-1, leu2-3, trp1-1, ura3, HO-ADE2, HO-CAN, she2::kanMX This study
YLM921b MATa, ade2-1, his3-11, leu2-3,112, trp1-1, ura3, ho, can1-100, she2Δ silent, she3::kanMX This study
YLM946b MATa, ade2-1, his3-11, leu2-3,112, trp1-1, ura3, ho, can1-100, trp1Δ silent, SHE3-myc13:TRP1 This study
YLM1309b MATa, ade2-1, his3-11, leu2-3,112, trp1-1, ura3, ho, can1-100, she2Δ silent, SHE1-myc13:kanMX6 This study
YLM1320b MATa, ade2-1, his3-11, leu2-3,112, trp1-1, ura3, ho, can1-100, she2Δ silent, she3Δ silent, SHE1-3HA: kanMX6 This study
YLM1368b MATa, ade2-, his3-11, leu2-3,112, trp1-1, ura3, ho, can1-100, trp1Δ silent, SHE3-myc13:TRP1, she2::kanMX This study
YLM1769d MATa, ade2, his3, leu2, trp1, ura3, mex67::HIS3, she2::kanMX, pUN100-mex67-5 This study

This Article

  1. RNA 9: 1383-1399