RNA–protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex

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FIGURE 2.
FIGURE 2.

She2p amino acid substitutions N36S and R63K do not affect the steady-state level of She2p. Yeast strain YLM777 was transformed with plasmids (lane 1) YCplac111 (vector), (lane 2) pRL199 (She2p-wt), (lane 3) pRL324 (She2p-N36S), or (lane 4) pRL322 (She2p-R63K). Transformants were grown in media devoid of leucine, and cell lysates were prepared. Cell lysates were used for Western blotting with anti-myc monoclonal antibody or anti-α-tubulin antibody.

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  1. RNA 9: 1383-1399

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