The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite–multisubstrate RNA:Ψ-synthase also acting on tRNAs

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FIGURE 1.
FIGURE 1.

Sequences and secondary structures of the Pus7p RNA substrates studied in this work. (A) The 5′-terminal part of S. cerevisiae U2 snRNA containing the three identified Ψ residues and the Sm site (Massenet et al. 1999). (B) The S. cerevisiae tRNAAsp (B1) and tRNAGlu (B2). (C) The S. cerevisiae (C1) and A. thaliana (C2) pre-tRNATyr are drawn with all their identified post-transcriptional modifications (tRNA database; Sprinzl et al. 1998; see also Internet site http://www.uni-bayreuth.de/departments/biochemie/sprinzl/trna/). The intronic sequences in pre-tRNAs are shown in small characters and arrows indicate exon–intron borders. The Ψ residues found to be formed by Pus7p are circled. Oligonucleotides used for primer extension analysis are indicated. In the yeast pre-tRNATyr transcript the C1–G72 base pair (boxed) was converted into a G1–C72 base pair to increase transcription efficiency.

This Article

  1. RNA 9: 1371-1382

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