The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5′-end processing of S. cerevisiae box C/D snoRNAs

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FIGURE 3.
FIGURE 3.

In vitro cleavage of snoRNA precursors model substrates by recombinant Rnt1p. In vitro transcribed unlabeled RNAs were incubated with buffer alone (lane 1), recombinant wild-type (lane 3), or mutant (E320K, lane 2) Rnt1p, and used as primer extension templates.

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  1. RNA 9: 1362-1370

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