The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5′-end processing of S. cerevisiae box C/D snoRNAs

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FIGURE 2.FIGURE 2.
FIGURE 2.

Primer extension analysis of 5′-end processing of box C/D snoRNAs in wild-type and rnt1Δ, xrn1Δ rat1-1, and rnt1Δ xrn1Δ rat1-1 mutant strains. (Lane 1) Wild-type strain, 25°C. (Lane 2) rnt1Δ strain, 25°C. (Lane 3) xrn1Δ rat1-1 strain, 25°C. (Lane 4) rnt1Δ xrn1Δ rat1-1 strain, 25°C. (Lane 5) Wild-type strain, shifted to 37°C. (Lane 6) rnt1Δ strain, shifted to 37°C. (Lane 7) xrn1Δ rat1-1 strain, shifted to 37°C. (Lane 8) rnt1Δ xrn1Δ rat1-1 strain, shifted to 37°C. In some cases, the oligonucleotide used cross-hybridized to another RNA species (indicated by an asterisk) on the figure. The mature species are indicated by “M”, and the precursors are indicated by “P”. Cleaved intermediates detected in the xrn1Δ rat1-1 strain, shifted to 37°C (lanes 7) are indicated by Cl-.

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  1. RNA 9: 1362-1370

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