FIGURE 7.
Enzymatic probing of UCCG and GUAG mutants. [γ-32P]ATP-5′ end-labeled RNA corresponding to domain 3 substitution mutants bearing the UCCG (A) or GUAG (B) sequence in the GNRA motif were incubated with RNase T1 and analyzed on 6% acrylamide 7 M urea gel electrophoresis. Asterisks denote the residues that showed enhanced sensibility to RNases, ss and ds stands for single stranded and double stranded, respectively. Filled arrows point to new digestion sites relative to the wild-type GUAA sequence.
