5′ Exon replacement and repair by spliceosome-mediated RNA trans-splicing

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FIGURE 5.
FIGURE 5.

Inserting an intron in the PTM coding unit results in a significant improvement in trans-splicing efficiency. The two PTMs shown in the panel above the histogram are identical, except that CF-PTM59 has a 543-bp CFTR mini-intron 9 in the LacZ coding unit. The 3′ LacZ exon in CF-PTM59 is 124 bp long. Values are mean ± standard error of two separate experiments. (BD) Binding domain.

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  1. RNA 9: 1290-1297

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