Combinatorial minimization and secondary structure determination of a nucleotide synthase ribozyme

Ribozyme da.7.02 kinetics at two different ^4sUra substrate concentrations. Timepoints were stopped in formamide loading dye and loaded on an APM polyacrylamide gel. (A) At 0.1 mM ^4SUra, the apparent first-order rate constant was 0.015 min⁻¹ (β = 0.10). (B) At 4 mM ^4SUra, the apparent first-order rate constant had risen to 0.21 min⁻¹ (β = 0.17). The fraction able to react, β, is dependent on ligation and transcription conditions together with the rate of pRpp hydrolysis.