Alignment of human Smg5/7a to Caenorhabditis elegans SMG5 and SMG7 and Drosophila melanogaster CG8954 and CG6369. (A) hSmg5/7a, C. elegans (Ce) SMG5, CeSMG7, and D. melanogaster (Dm) CG8954, and DmCG6369 are diagrammed as horizontal bars. The number of constituent amino acids (aa) is provided to the right of each bar. Conserved regions 1 (C1) and 2 (C2) are boxed and aligned with dashes. (B) Amino acids 721–816 of hSmg5/7a, 136–214 of CeSMG7, and 279–375 of DmCG6369 are aligned at C1; amino acids 1241–1276 of hSmg5/7a, 422–464 of CeSMG5, 765–815 of DmCG6369, and 1008–1055 of DmCG8954 are aligned at C2. White letters in black boxes specify amino acids that are either identical or similar among all compared proteins. White letters in gray boxes specify amino acids that are either identical or similar among only two (C1) or three (C2) compared proteins. Black letters in gray boxes specify amino acids that are either identical or similar between only two (C2) compared proteins. (C) Percent (%) identity/similarity between proteins within C1 or C2.
