A 100-kD complex of two RNA-binding proteins from mitochondria of Leishmania tarentolae catalyzes RNA annealing and interacts with several RNA editing components

Binding of Ltp26/Ltp28 complex to T7 transcribed gRNA. (A) Binding of Ltp26/Ltp28 100-kD complex to RNA. In vitro transcribed labeled gRPS12-I gRNA was incubated with purified Ltp26/Ltp28 complex in 100 μL of 20 mM HEPES, pH 7.5, 5 mM MgCl₂, 150 mM KCl, and separated on a Superose 6 column. The peak on left shows 50 pmole of gRNA and 500 pmole of protein, and the large peak on right 50 pmoles Ltp26/Ltp28 and 500 pmoles gRNA. (B) Radiolabeled gRPS12-I gRNA (20 nM) was incubated with increasing concentrations of Ltp26/Ltp28 complex under the same conditions in 5 μL aliquots and separated on 8%–16% native Tris-glycine gel.