A 100-kD complex of two RNA-binding proteins from mitochondria of Leishmania tarentolae catalyzes RNA annealing and interacts with several RNA editing components

Reconstitution of Ltp26/Ltp28 100-kD complex. (A) Superose 12 gel filtration profiles of purified Ltp26/Ltp28 complex, of the material reassembled from the purified recombinant Ltp26 and Ltp28 proteins, and of the individual proteins. Positions of molecular weight markers are indicated. (B) Protein composition of native and reconstructed complexes. Fractions were collected from the same starting point in chromatographic runs (above) and separated on 10%–20% SDS gradient gels which were stained with Sypro Ruby.