A 100-kD complex of two RNA-binding proteins from mitochondria of Leishmania tarentolae catalyzes RNA annealing and interacts with several RNA editing components

Interaction of Ltp26 and Ltp28 proteins. (A) Chemical crosslinking of Ltp26/Ltp28 complex. Purified Ltp26/Ltp28 complex was incubated with homobifunctional protein–protein crosslinking reagents, and the products were separated on an SDS gel which was stained with Sypro Ruby. (B) Yeast two-hybrid analysis of the interaction between Ltp26 and Ltp28. Samples of cotransformed yeast grown on medium lacking tryptophan, leucine, and histidine. (C) Coimmunoprecipitation of epitope-tagged proteins synthesized in vitro in a coupled transcription/translation system. All IPs were with a monoclonal anti-Myc antibody. Lane 1: ³⁵S-labeled Ltp26 tagged with HA. Lane 2: ³⁵S-labeled Ltp26 tagged with HA mixed with unlabeled Ltp28 tagged with Myc. Lane 3: ³⁵S-labeled Ltp28 tagged with Myc. Lane 4: ³⁵S-labeled Ltp26 tagged with HA mixed with unlabeled TUTase tagged with Myc. Lane 5: ³⁵S-labeled TUTase tagged with Myc.