Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure

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FIGURE 1.
FIGURE 1.

Overview and model of DsrA/Hfq/RpoS-mediated regulation. (A) Overview of the role of DsrA in H-NS and RpoS-mediated translational regulation. DsrA increases the translation of RpoS mRNA, leading to increased transcription of RpoS-regulated genes. DsrA decreases the translation of H-NS, leading to increased transcription of H-NS-repressed genes (Sledjeski et al. 1996). (B) Model of DsrA/Hfq/RpoS interaction. In this model, Hfq unfolds the RpoS 5′-leader region, allowing DsrA Domain I to base-pair with the RpoS mRNA (Majdalani et al. 1998). This stabilizes an alternative conformer of RpoS mRNA that leads to increased translation by exposing the ribosome-binding site. (C) Sequence and proposed secondary structure of DsrA based on Lease and Belfort (2000).

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  1. RNA 9: 33-43

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