TABLE 1.
Interactions identified in the yeast two-hybrid screensa
| Protein | ORF | Protein length (amino acids) | MRIb | Isolatesc | Categoryd |
|---|---|---|---|---|---|
| aThe open reading frames that were identified as prey in two-hybrid screens with full-length Prp22p, Prp45p, and Prp46p bait fusions. Indicated are systematic protein and ORF names as given in the Saccharomyces Genome Database. | |||||
| bMRI: minimal region of interaction—amino acid boundaries of the region shared by all isolates of a given protein (* C termini of these inserts have not been determined). | |||||
| cIsolates: total number of prey isolates. | |||||
| dCategory: Prey fusions were classified as defined by Fromont-Racine et al. (1997). A1 (statistically the most significant): multiple coding fusions that share a common region; A2: fusion starts close to the translation initiation codon; A3: contains a large coding insert; A4: single coding fusion not represented in A2 or A3 category, and possibly the least significant, although this may also indicate that only a precisely defined peptide can mediate an interaction. | |||||
| Prp22p two-hybrid screen isolates | |||||
| Prp45p | YAL032c | 379 | 262–291 | 10 | A1 |
| Syf3p | YLR117c | 687 | 628–687 | 5 | A1 |
| YNL274c | 350 | 33–350 | 1 | A3 | |
| Pmi40p | YER003c | 460 | 342–460 | 1 | A4 |
| Dbp7p | YKR024c | 742 | 327–621 | 2 | A4 |
| Prp45p two-hybrid screen isolates | |||||
| YDR141c | 1698 | 580–* | 2 | A1 | |
| Fir1p | YER032w | 925 | 709–831 | 4 | A1 |
| YGR198w | 817 | 748–817 | 3 | A1 | |
| Yta7p | YGR270w | 1379 | 835–1198 | 4 | A1 |
| Ubr2p | YLR024c | 1872 | 834–* | 4 | A1 |
| Syf3p | YLR117c | 687 | 35–156 | 6 | A1 |
| Vac14p | YLR386w | 880 | 331–* | 5 | A1 |
| Sgs1p | YMR190c | 1447 | 503–739 | 2 | A1 |
| Prp46p | YPL151c | 451 | 127–432 | 19 | A1 |
| Cdc27p | YBL084c | 758 | 30–412 | 2 | A2 |
| YLR424w | 708 | 31–* | 1 | A2/A3 | |
| YMR031c | 843 | 1–* | 1 | A2 | |
| Vps75p | YNL246w | 264 | 26–* | 1 | A2 |
| Ahc1p | YOR023c | 566 | 19–* | 1 | A2 |
| Pkc1p | YBL105c | 1151 | 301–* | 1 | A3 |
| Syf1p | YDR416w | 859 | 442–827 | 2 | A3 |
| Ade6p | YGR061c | 1358 | 792–* | 1 | A3 |
| YGR071c | 860 | 301–* | 1 | A3 | |
| Sec6p | YIL068c | 806 | 100–* | 1 | A3 |
| Apl1p | YJR005w | 700 | 159–* | 1 | A3 |
| YJR061w | 935 | 395–* | 1 | A3 | |
| Dyn1p | YKR054c | 4092 | 690–* | 2 | A3 |
| Mms22p | YLR320w | 1454 | 611–* | 2 | A3 |
| Nup188p | YML103c | 1655 | 1291–* | 1 | A3 |
| Ecm5p | YMR176w | 1411 | 790–* | 1 | A3 |
| Sla2p | YNL243w | 968 | 114–* | 1 | A3 |
| Ski3p | YPR189w | 1432 | 463–* | 1 | A3 |
| Rad16p | YBR114w | 790 | 59–* | 1 | A4 |
| YDL001w | 430 | 362–* | 1 | A4 | |
| Nup84p | YDL116w | 726 | 170–* | 1 | A4 |
| Ngg1p | YDR176w | 702 | 508–* | 1 | A4 |
| Trp5p | YGL026c | 707 | 617–* | 1 | A4 |
| Yap1801 | YHR161c | 637 | 112–* | 1 | A4 |
| YJL103c | 618 | 219–* | 1 | A4 | |
| YJL149w | 663 | 508–* | 1 | A4 | |
| YLR187w | 1026 | 167–* | 3 | A4 | |
| Nis1p | YNL078w | 407 | 270–* | 1 | A4 |
| Bni1p | YNL271c | 1953 | 1481–* | 1 | A4 |
| Mnt4p | YNR059w | 580 | 195–* | 1 | A4 |
| Nif1p | YOR156c | 726 | 361–* | 1 | A4 |
| Eaf3p | YPR023c | 401 | 228–* | 1 | A4 |
| Prp46p two-hybrid screen isolates | |||||
| Prp45 | YAL032c | 379 | 54–204 | 5 | A1 |
| Syf3 | YLR117c | 687 | 10–243 | 2 | A1 |
| HFM1 | YGL251c | 1046 | 699–880 | 2 | A1 |
| Pkc1 | YBL105c | 1151 | 301–801 | 1 | A3 |
| YLR426w | 202 | 202 | 1 | A2 | |
| VAP | YBR069c | 619 | 290–574 | 1 | A4 |
| PEP12 | YOR036w | 288 | 288 | 1 | A4 |
