Sequence requirements for micro RNA processing and function in human cells

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FIGURE 7.
FIGURE 7.

Two miRNAs can be expressed from a single transcript. Schematic of the CMV-IE-based pCMV/miR-30-miR-30-E7 and H1 promoter-based pH1/miR-30-miR-30-E7 miRNA expression plasmids. Transfected 293T cells produced both mature miR-30 and miR-30-E7, as detected by primer extension (shown by arrows). −, RNA from mock transfected cells.

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