FIGURE 3.
Analysis of miR-21 mutants. (A) Primer extension to detect mature miR-21 (lanes 1–9) or anti-miR-21 (lanes 10–12) in transfected cells. −, Cells transfected with pBC12/CMV (lane 1); +, 20 pg of a DNA oligonucleotide encoding mature miR-21 (lane 9) or the predicted anti-miR-21 (lane 12). Arrows indicate the expected position of the mature miRNA. (B) Schematic of the CAT indicator plasmid containing four miR-21 target sites (black boxes). The predicted partial base-pairing between miR-21 and its artificially designed target site, and the positions of two miR-21 point mutations, miR-21(C3) and miR-21(U), are shown. (C) CAT assay. See Figure 2B legend for details.
