FIGURE 2.
Biological activity of miR-30 mutants. (A) Schematic of the indicator construct with four copies of a miR-30 target site (black boxes). The predicted base-pairing between miR-30 and the mRNA target, and the position of three miR-30 mutations are shown. (B) CAT expression in 293T cells cotransfected with the indicator plasmid pcRev and various miR-30 expression plasmids, as well as with the pBC12/CMV/β-gal internal control plasmid. CAT activities were normalized using the β-gal activities, and set at 1 for cells transfected with the pBC12/CMV negative control plasmid (marked as −). Error bars represent standard deviations.
