TABLE 1.
The kinetic analyses of the T. thermophilus ValRS and tRNAVal (CAC) mutants
| ValRS Transcript | KM(tRNA) (μM) | kcat (sec−1) | kcat/KM (sec−1 • μM−1) (relative) |
|---|---|---|---|
| Wild-type | 1.5 ± 0.44 | 6.7 ± 2.0 | 4.4 (1) |
| Δ(795–862) | 42 ± 8.2 | 0.36 ± 0.16 | 8.5 × 10−3 (1.9 × 10−3) |
| R818A/R843A | 31 ± 14 | 4.3 ± 1.3 | 1.4 × 10−1 (3.2 × 10−2) |
| ValRS Transcript | KM(tRNA) (μM) | kcat (sec−1) | kcat/KM (sec−1 • μM−1) (relative) |
|---|---|---|---|
| Wild-type | 0.70 ± 0.21 | 1.3 ± 0.40 | 1.9 (1) |
| Δ(795–862) | 36 ± 1.6 | 1.8 × 10−2 ± 9.4 × 10−3 | 5.0 × 10−4 (2.6 × 10−4) |
| R818A/R843A | 18 ± 7.3 | 1.1 ± 0.12 | 6.1 × 10−2 (3.2 × 10−2) |
| ValRS Transcript | KM(tRNA) (μM) | kcat (sec−1) | kcat/KM (sec−1 • μM−1) (relative) |
|---|---|---|---|
| Wild-type | 1.5 ± 0.44 | 6.7 ± 2.0 | 4.4 (1) |
| G18U | 13 ± 5.6 | 4.2 ± 1.1 | 3.2 × 10−1 (7.2 × 10−2) |
| G19C | 27 ± 1.5 | 1.2 ± 0.3 | 4.4 × 10−2 (1.0 × 10−2) |
| G18U/G19C | 31 | 0.21 | 6.8 × 10−3 (1.5 × 10−3) |
| G19C/C56G | 5.5 ± 0.4 | 5.3 ± 0.1 | 9.6 × 10−1 (2.2 × 10−1) |
